Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells.Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex and Dermatopathia pigmentosa reticularis, both of which are autosomal dominant mutations.KRT14 belongs to a large group of acidic type I keratins that interact with basic type II keratins to form the 8-nm cytoskeletal filaments of epithelial cells. Both type I and type II keratins have a central alpha-helical domain of over 300 amino acids that mediates keratin interaction. KRT14 is expressed in the basal layer of stratified squamous epithelia, including epidermis .
Human Keratin, type I cytoskeletal 14 (KRT14) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT14 in samples. An antibody specific for KRT14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Keratin, type I cytoskeletal 14 (KRT14) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-keratin-type-i-cytoskeletal-14-krt14-elisa-kit-kte61884/
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