Cytokeratin 20(CK20) is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract. Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium.CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin.
Human Cytokeratin 20 (CK20) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT20 in samples. An antibody specific for KRT20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Cytokeratin 20 (CK20) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-cytokeratin-20-ck20-elisa-kit-kte61880/
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