Human Keratin, type I cuticular Ha3-I (KRT33A) ELISA Kit,Abbkine_specification/price/image

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KRT33A is a member of the keratin gene family. It is one of the type I hair keratin genes which are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B.Rogers et al. (1998) reported that the deduced KRTHA3A protein has 405 amino acids and shares 93.3% amino acid sequence identity with KRTHA3B. By RT-PCR, Rogers et al. (1998) showed that KRTHA3A is expressed in the human hair follicle. See Langbein et al. (1999) for further details on the expression pattern of the KRTHA3A gene in the hair follicle.

Human Keratin, type I cuticular Ha3-I (KRT33A) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT33A in samples. An antibody specific for KRT33A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT33A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT33A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT33A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Keratin, type I cuticular Ha3-I (KRT33A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.