Keratin 7 is a member of the keratin family. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Human Keratin, type II cytoskeletal 7 (KRT7) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT7 in samples. An antibody specific for KRT7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Keratin, type II cytoskeletal 7 (KRT7) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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