Human Keratin, type II cuticular Hb1 (KRT81) ELISA Kit,Abbkine_specification/price/image

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KRT81 is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The predicted 495-amino acid HB1 protein has an 88-amino acid C-terminal domain. The amino acid sequences of human and sheep HB1 are 90% identical. Northern blot analysis detected HB1 transcripts in human scalp but not unhaired breast epidermis.HB1 is more closely related to HB3 and HB6 than to HB5. In situ hybridization studies demonstrated that type II keratin genes are sequentially activated in the hair follicle in the following order: HB5--(HB1, HB3)--HB6. Both HB1 and HB3 mRNA syntheses begin 10 to 15 cell layers above the apex of the dermal papilla and end abruptly in the middle of the cortex.

Human Keratin, type II cuticular Hb1 (KRT81) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT81 in samples. An antibody specific for KRT81 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT81 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT81 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT81 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Keratin, type II cuticular Hb1 (KRT81) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-keratin-type-ii-cuticular-hb1-krt81-elisa-kit-kte61872/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.