Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit,Abbkine_specification/price/image

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The MCCD1 gene encodes a deduced 119-amino acid protein containing a putative coiled-coil domain at its C terminus and a predicted mitochondrial localization signal at its N terminus. RT-PCR analysis detected expression of MCCD1 in all human tissues tested, at highest levels in adult and fetal kidney, liver and lung, and fetal brain, and at lower levels in fetal spleen.The human and pig proteins share 65.9% overall sequence identity but their C-terminal domains are highly conserved, showing 92% identity over 53 residues. No orthologs were identified in any other species, suggesting that the gene arose relatively recently in evolution. Transient expression in mammalian cells of MCCD1 fused at its C terminus to either EGFP or the T7-epitope tag showed that the protein is localized to mitochondria.

Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCCC1 in samples. An antibody specific for MCCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCCC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.