Mps one binder kinase activator-like 1A encoded by MOBKL1A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for MOBKL1A.
Human Mps one binder kinase activator-like 1A (MOBKL1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MOBKL1A in samples. An antibody specific for MOBKL1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMOBKL1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOBKL1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOBKL1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mps one binder kinase activator-like 1A (MOBKL1A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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