Mammalian target of rapamyein encoded by MTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of MTOR. MTOR (Mechanistic Target Of Rapamycin) is a Protein Coding gene. Diseases associated with MTOR include Smith-Kingsmore Syndrome and Macrocephaly-Intellectual Disability-Neurodevelopmental Disorder-Small Thorax Syndrome. Among its related pathways are CXCR4-mediated signaling events and RET signaling. GO annotations related to MTOR include transferase activity, transferring phosphorus-containing groups and protein serine/threonine kinase activity. An important paralog of this gene is SMG1.
Human Mammalian target of rapamyein (MTOR) ELISA Kit employs a two-site sandwich ELISA to quantitate MTOR in samples. An antibody specific for MTOR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTOR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTOR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mammalian target of rapamyein (MTOR) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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