The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. MUC4 encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of MUC4 have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. MUC4 contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats.
Human Mucin-4 (MUC4) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC4 in samples. An antibody specific for MUC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mucin-4 (MUC4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-mucin-4-muc4-elisa-kit-kte61454/
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