Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit,Abbkine_specification/price/image

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The carboxyterminal propeptide of type I procollagen (PICP) and the aminoterminal propeptide of type I procollagen (PINP) are enzymatically removed from the procollagen molecule by specific proteases.PICP and PINP are indicators of the synthesis of type I collagen. Cleavage of PICP is required for the initiation of fibril formation, whereas PINP may be retained in the collagen molecule resulting in type I pN-collagen. The retention of PINP is transient, unlike the presence of IIIpN-collagen, and has been considered to regulate fibril diameter (Fleischmajer et al. 1985).PICP is a trimeric, globular protein consisting of three polypeptide chains: two proα1(I) and one proα2(I) chains. The molecular mass of PICP is 100 000 and it is cleared by mannose receptors in liver endothelial cells (Smedsrød et al. 1990).

Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit employs a two-site sandwich ELISA to quantitate PINP in samples. An antibody specific for PINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PINP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.