Human Platelet-activating factor acetylhydrolase 2, cytoplasmic (PAFAH2) ELISA Kit,Abbkine_specification/price/image

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Platelet-activating factor acetylhydrolase 2, cytoplasmic is a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes.Additionally, there is a single-subunit serum isoform of this enzyme.PAFAH2 shares approximately 43% identity with plasma PAFAH, but has virtually no similarity to brain PAFAH1B1. Unlike PAFAH, PAFAH2 contains no secretory signal sequence, confirming its intracellular localization.

Human Platelet-activating factor acetylhydrolase 2, cytoplasmic (PAFAH2) ELISA Kit employs a two-site sandwich ELISA to quantitate PAFAH2 in samples. An antibody specific for PAFAH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAFAH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAFAH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAFAH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Platelet-activating factor acetylhydrolase 2, cytoplasmic (PAFAH2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.