Cadherins are calcium-dependent cell-cell adhesion molecules, and protocadherins constitute a subfamily of nonclassic cadherins. PCDHGA2 is 1 of 22 tandemly arranged genes within the PCDHG gene cluster on chromosome 5q31. The 22 PCDHG genes function as 'variable' exons that are individually spliced to a downstream constant region to form distinct PCDHG transcripts. The variable PCDHG exons encode the extracellular and transmembrane domains of the protocadherin protein, and the common region encodes the intracellular domain. For further information on the PCDHG.By genomic sequence analysis, Wu et al. (2001) mapped the PCDHGA2 gene to 5q31, between the PCDHGA1 gene and the PCDHGA3 gene. They localized the mouse PCDHG genes to chromosome 18c.
Human Protocadherin gamma-A2 (PCDHGA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHGA2 in samples. An antibody specific for PCDHGA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDHGA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDHGA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHGA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Protocadherin gamma-A2 (PCDHGA2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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