Human Neuroendocrine convertase 2 (PCSK2) ELISA Kit,Abbkine_specification/price/image

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Proprotein convertase 2 is a serine protease and proprotein convertase PC2, like proprotein convertase 1 (PC1), is an enzyme responsible for the first step in the maturation of many neuroendocrine peptides from their precursors, such as the conversion of proinsulin to insulin intermediates. To generate the bioactive form of insulin (and many other peptides), a second step involving the removal of C-terminal basic residues is required; this step is mediated by carboxypeptidases E and/or D. PC2 plays only a minor role in the first step of insulin biosynthesis, but a greater role in the first step of glucagon biosynthesis compared to PC1. PC2 binds to the neuroendocrine protein named 7B2, and if this protein is not present, proPC2 cannot become enzymatically active.

Human Neuroendocrine convertase 2 (PCSK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK2 in samples. An antibody specific for PCSK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Neuroendocrine convertase 2 (PCSK2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-neuroendocrine-convertase-2-pcsk2-elisa-kit-kte61287/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.