Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit,Abbkine_specification/price/image

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CNK is a member of the 'polo' family of serine/threonine kinases. Members of this family have been implicated in cell division and are important in regulating the onset of mitosis and M-phase progression.The predicted 607-amino acid protein has an N-terminal catalytic domain, including an ATP-binding site, a central putative nuclear targeting signal, and a presumed C-terminal regulatory domain. The CNK protein shows homology with several other members of the 'polo' family; it is 91% identical to mouse Fnk, 50% identical to human PLK, 48% identical to Drosophila polo, and 38% identical to S. cerevisiae Cdc5. Northern blot analysis detected expression of a 2.5-kb CNK transcript in a limited number of human tissues, with placenta containing a moderate level and ovary, lung, and peripheral blood leukocytes containing low levels.

Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate PLK3 in samples. An antibody specific for PLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.