Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) ELISA Kit,Abbkine_specification/price/image

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PPIAL4G belongs to the cyclophilin-type PPIase family. PPIase A subfamily. Contains 1 PPIase cyclophilin-type domain, PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIases are ubiquitous in living organisms. While 3 families of PPIases, cyclophilin (CyP), FK506 binding protein (FKBP) and parvulin (Pvn), have been studied in detail in Eukarya and Bacteria (eubacteria), little is known about archaeal PPIases. Among 2 cyclophilins found in Archaea, only Halobacterium cyclophilin (HcCyP19) has been characterized. It is a cyclosporin A (CsA) sensitive CyP with a MW of 19.4kDa. The PPIase activity and CsA sensitivity of this CyP is higher at higher salt concentration in the medium. No parvulin or its homolog has been found in Archaea.

Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) ELISA Kit employs a two-site sandwich ELISA to quantitate PPIAL4G in samples. An antibody specific for PPIAL4G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPIAL4G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPIAL4G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPIAL4G bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.