Recombinant Trypsin

Catalog Number: RPT0201

CAS: 9002-07-7
EC: 3.4.21.4

Storage temperature: 2-8℃

Source: Recombinant trypsin is a genetically engineered protein expressed in E.coli 

Protein Sequence:The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin

1.DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.

2.MAIN FEATURES

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A₂₅₃ of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

3.RECOMMEND USAGE

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0. 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant trypsin lyophilized should be stored under 2-8℃ in sealed container.

 It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃. 

It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

1. Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
2. Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
3. High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
4. Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
5. Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
6. Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

6.PRODUCT USAGE

The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as in insulin and vaccine production.

7.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase