AMSBIO: High Throughput PARP Screening Assay

AMSBIO is a specialist life science company offering an extensive portfolio of Poly (ADP-ribose) polymerase (PARP) isozymes. In addition, AMSBIO also offer assay kits, inhibitors, recombinant histone substrates and screening / profiling services for over 10 different PARP’s.

Poly (ADP-ribose) polymerase (PARP) is a family of proteins that transfer ADP-ribose units from NAD+ onto target nuclear proteins, forming long branched Poly ADP-ribose (PAR) chains. PARPs play key signaling roles in apoptosis, DNA excision and repair, and chromatin structure. PARPs are also involved in transcriptional regulation of several signaling pathways, including genes involved in inflammation.

The latest addition to the AMSBIO range is the PARPtrap™ Assay Kit which is designed to measure PARP1/DNA complex formation in a high throughput screening assay using fluorescence polarization.

The PARPtrap™ Assay Kit comes in a convenient 96-well format, with purified PARP1 enzyme, fluorescent labelled nicked DNA, and PARPtrap™ assay buffer for 100 enzyme reactions. The key to the PARPtrap™ Assay Kit is the fluorescent labelled nicked DNA. Without the PAR reaction, PARP1 binds to the fluorescent labelled nicked DNA, resulting in the emission of highly polarized light. However, after autoribosylation of PARP1, the nicked DNA is dissociated from PARP1 and rotates freely, emitting less polarized light.

Designed for research use the  PARPtrap™ Assay Kit  sets a new standard for screening small molecules that enhance PARP1/DNA trapping in drug discovery and high throughput screening applications.

For further information on the new PARPtrap™ Assay Kit please visit  http://www.amsbio.com/parp-assay-kits-enzymes.aspx or contact AMSBIO on 44-1235-828200/ +1-617-945-5033 / info@amsbio.com.

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products and services for research and development in the medical, nutrition, cosmetics and energy industries. AMSBIO can draw upon in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. Widely acknowledged as an expert in cell culture, AMSBIO partners with clients in tailoring cell systems to enhance organoid and spheroid type screening outcomes from a technological and cost-effective perspective.

Worldwide HQ 

AMS Biotechnology (AMSBIO)

184 Milton Park
Abingdon

Oxon OX14 4SE

UK
Tel: +44-1235-828200
Fax: +44-1235-820482
Email: info@amsbio.com 
Web www.amsbio.com

 

North American HQ

 

AMSBIO LLC
1035 Cambridge Street
Cambridge
MA 02141
USA
Tel: +1.617.945.5033 
Tel: +1.800.987.0985 (toll free)
Email:  info@amsbio.com
Web www.amsbio.com