AMSBIO: Consistent Embryoid Body Formation and Differentiation from Stem Cells

AMSBIO, experts in extracellular matrices, have introduced the Cultrex® Embryoid Body Formation Kit to generate consistent, reproducible and identical in size embryoid bodies. It has been qualified to form embryoid bodies from human pluripotent stem cells. Embryoid bodies are finding increasing use in regenerative medicine, in-vitro screening and as a model of embryonic development.

The new kit includes ROCK Inhibitor Y-27632, which significantly increases survival of PSCs in single cell culture, a specialized Embryoid Body Formation Matrix to stimulate cell aggregation and development of embryoid bodies, and a 96-well Embryoid Bodies Formation Plate, that allows for control of the embryoid body size and shape. No transfer step is required for differentiation.

Cultrex® Embryoid Body Formation Kit helps to generate identical sized EBs. A, B, C - EBs formed from human iPSCs using Cultrex® Embryoid Body Formation Kit; showing EBs generated with 3,000 cells per well after 48 hours. D, E, F - EBs formed from human iPSCs using conventional method; 3,000 cells per well after 48 hours. Each image (A-F) represents individual well of 96-well plate

Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have the unique ability to differentiate into cells of three embryonic germ layers (endoderm, ectoderm and mesoderm). The majority of differentiation protocols start with the generation of embryoid bodies – three-dimensional multicellular aggregates of pluripotent stem cells. Although pluripotent stem cells placed in suspension culture will form embryoid bodies spontaneously, the efficiency of this process varies and is not reproducible. Most current methods avoid preparing single cell suspension, due to low survival of pluripotent stem cells in single cell culture, and require keeping cells in large clumps, thus generating embryoid bodies of different size and shapes. Size of embryoid bodies is one of the key factors effecting differentiation of PSCs and needs to be controlled.

Currently the Cultrex® Embryoid Body Formation Kit from AMSBIO is the only commercially available kit for formation and differentiation of size-consistent embryoid bodies. For further information please visit http://amsbio.com/Embryoid-Body-Formation.aspx or contact AMSBIO on on+44-1235-828200 (Europe / ROW) / +1-800-987-0985 (North America) or email info@amsbio.com. 

Founded in 1987, AMS Biotechnology (AMSBIO) is recognised today as a leading company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products and services for research and development in the medical, nutrition, cosmetics and energy industries. AMSBIO is able to draw upon in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. Widely acknowledged as an expert in cell culture, AMSBIO
partners with clients in tailoring cell systems to enhance organoid and spheroid type screening outcomes from a technological and cost-effective perspective. Custom services include stable cell line production, CRISPR/Cas9 Genome Editing, lentivirus and adenovirus production, large scale protein production and a comprehensive portfolio of antibody services.

---------------------

Worldwide HQ 

AMS Biotechnology (AMSBIO)

184 Milton Park
Abingdon

Oxon OX14 4SE

UK

Tel: +44-1235-828200
Fax: +44-1235-820482
Email: info@amsbio.com 
Web www.amsbio.com

 

 

North American HQ

 

AMSBIO LLC
1035 Cambridge Street
Cambridge
MA 02141
USA

Tel:      +1.617.945.5033 
Tel:      +1.800.987.0985 (toll free)
Email: info@amsbio.com 
Web www.amsbio.com