1. Intended Use
A simple and efficient extraction and purification procedure for Folic acid was developed by means of the immunoaffinity column (IAC - SEP ® FA) as a cleanup tool. This method do not use toxic solvents such as chloroform and methylene chloride. Folic acid content in drink, milk, milk powder are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring Folic acid.
2. Principle
Samples are prepared by mixing with an extraction solution(or just centrifuging), blending and filtering.The extract is then applied to the Folic acid immunoaffinity column bound with specific antibodies to Folic acid．At this stage, the Folic acid bind to the antibody on the column.The column is then washed with water to remove the impurities. By passing 30 % Acetonitrile Containing 0.2 % TFA through the column, the Folic acid is removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.
Wuhan Ecalbio Co., Ltd
Web: www.ecalbio.com
Address: HZAU, Nanhu Road, Wuhan city 430075, China.
Tel: +86 27 87745856
Fax: +86 27 87745856
E-mail: info@ecalbio.com
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Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.
Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.
Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.
Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.
Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
Government Laboratories
Food industry Laboratories
Institutes
Veterinary Clinics
Pharmaceutical Industry.
Ecalbio welcomes potential partners and distributors to explorebusiness relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.
ECALBIO CO.,LTD
HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com
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