Product Overview
Large-Scale Transfection Using the 4D-Nucleofector™ LV Unit - Experience the new functional unit for the 4D-Nucleofector™ System which expands our proven system to larger-scale transfection. The LV Unit allows for closed, scalable transfection of larger cell numbers in the range of 1x107 to 1x109 cells. Transfection protocols can be established in smaller scale using the X Unit and subsequently transferred to the LV Unit without the need for re-optimization. Transferability has been tested for various cell types, including human T cells, CHO-S, HEK293-S, or K-562.

 

Technical Specifications
Dimensions: (w x h x d): 24.5×20.7×28 cm / 9.7×4.1×11.0 
Weight: 5.7 kg (12.5 lb) 
Power: 100 - 240 VAC 
Frequency: 50 - 60 Hz 
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection) 
Fuse: T 2.5A, L250V 
Operating temperature: +15°C up to +40°C (59°F - 104°F) 
Environnement non condensing Voltage limitation: 1500 V 
Current limitation: 100 A

Benefits
Closed system: Sterile Nucleofection of up to 109 cells
Real scalability: Optimization in small scale 
Established protocols: Benefit from 700+ optimized cell types
Simple handling: Minimal training needs
4D-Nucleofector™ LogWare: Optional operation via 21CFR part11 compliant software

Applications
Ex-vivo modification of human primary cells for cell therapy applications (e.g. genome editing, generation of CAR-T cells)
Transient production of potential therapeutic proteins or antibodies for construct screening
Generation of large numbers of transiently modified primary cells for cell-based assays

Content & Storage
Content
1 Unit

As a worldwide leader supplying the pharmaceutical and biotechnology industries with biopharmaceuticals, Lonza furnishes different markets with state-of-the-art products, services, and research. Our customers range from professionals within the pharmaceutical, biotechnology, academic, and government research industries to manufacturers of consumer and health products, distributors, formulators, and service companies.

Lonza’s Market Offerings:

BioResearch: A leader in groundbreaking research and quality control laboratory testing systems that include tools for endotoxin detection and for the discovery of therapeutics.

Pharma & Biotech: An established supplier to the pharmaceutical industry with global manufacturing and development expertise, advanced technologies and high-quality products.

Consumer Care: A global market leader that draws on science to promote health, wellness, beauty and protection in the areas of Personal Care, Hygiene and Nutrition

Water Treatment: One of the leading suppliers of treatment chemicals, sanitizer feeder systems and other services to the global residential, industrial, commercial and municipial water treatment markets.

Wood Protection: A leading name in industrial pretreatment and surface protection of wood, with the world’s broadest portfolio of wood-protecting chemistries and wood-preservation technologies.

Agro Ingredients: A trusted supplier for the world’s largest agricultural companies with a wide range of offerings, such as custom manufacturing of active ingredients and agrochemical intermediates.

Industrial Solutions: A diverse supplier with a broad chemical portfolio and a common goal of helping to conserve energy and supply cost-effective, sustainable solutions to the Oil & Gas, Paints & Coatings, Electronics, Aerospace and Building Products markets.