1. Sample Preparation
The candidate of reference material was prepared by biological zymolysis methods and purify by preparative chromatography technique.

Compound	Molecular formula	CAS
Patulin (PAT)	C7H6O4	149-29-1

 
2 . Traceability and Characterization Method
Candidate material was characterized by UV spectroscopy, melting point method, and nuclear magnetic resonance. Mass balance and qNMR methods was used to assign value of the RM. In mass balance, high performance liquid chromatography with diode array detector (HPLC-PDA)  was used to determine the main component. Water was determined by Karl-fisher method. Total non-volatiles/inorganics were determined by ICP-MS and TGA method. Volatile solvents were determined by qNMR and GC-ECD methods.
 
3. Certified values and uncertainties
The property values and expanded uncertainty of the present CRM are as follows：

No.	Reference material	Certified Value (%)	U (%) k=2
GBW10257	Patulin (PAT)	99.7	0.2

 

---

ECALBIO CO., LTD
Tel: 86-27-87745856
Email:info@ecalbio.com
HZAU, Guanshan Road , Wuhan City, China
 

Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.

Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.

Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.

Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
　Government Laboratories
　Food industry Laboratories
　Institutes
　Veterinary Clinics
　Pharmaceutical Industry.

Ecalbio welcomes potential partners and distributors to explorebusiness relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.

ECALBIO CO.,LTD

HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com