Recombinant Proteinase K
Catalog Number:RPK09
Cas:39450-01-6
EC:3.4.21.14
Alternate names:Protease K, Endopeptidase K, Tritirachium alkaline proteinas
DESCRIPTION
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
ADVANTAGES
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Format
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Lyophilized
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M.W
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29.3kD
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Purity
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99%
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Activity
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30 units/mg
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DNAses and RNAses
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not detected
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UNIT DEFINITION
One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).
RECOMMEND USAGE
Prepare recombinant Proteinase K with 50 mM Tris-HCl,2 mM Calcium acetate.The optimum pH is 8.0.
STORAGE INSTRUCTIONS
Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.
HANDLING
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
bio-equip.cn
Shanghai Yaxin Biotechnology Co. Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins. Shanghai YaxinBio,located in Shanghai Juke Biotech Park, is ISO 9001 registered company since 2009.
Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.YaxinBio recombinant carboxypeptidase B and recombinant trypsin have no trace of other contaminating enzyme activities or enzyme inhibitors. They have been successfully used in the production process of recombinant insulin by several companies. Recombinant Trypsin can also be used in the process of cell culture.
Shanghai YaxinBio continuously strives to bring to its worldwide customers the best recombinant protein products. We will continue strengthen our product management and our internal system to meet the demands of biotech companies and scientists in the field of life sciences.
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