Protein A
Protein A is a 40-60 kD surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgG’s. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. The result of this type of interaction is that, in serum, the bacteria will bind IgG molecules in the wrong orientation (in relation to normal antibody function) on their surface which disrupts opsonization and phagocytosis.
Product: ProteinA , recombinant, expressed in E. coli
M W: 35KD (SDS-PAGE)
Puritey: >95%（Determined by HPLC, Chromatographic purification
Binding Capacity (Human IgG): >5mg IgG /mg proteinA, approx. 7mg IgG/mg
Solution: 20mM PBS Poly(butylenes succinate pH7.4
USE: ELISA capture reagent；Conjugating reagent for protein A affinity column for IgG purification; etc

Beijing Hotgen Biotech Co.,Ltd, which is located in Beijing Zhongguancun High-tech
development zone, is a high-tech enterprise. We offer IVD raw materials, diagnostic
reagents, and OEM service as well.
Hotgen Biotech is one of the main suppliers of IVD biological raw materials in China. The
raw materials, including recombinant antigens and monoclonal antibodies, have been
exported to North America. Our products are popular due to high quality, and little
variation between each batch.