Recombinant Human Basic Fibroblast Growth Factor (bFGF /FGF2)

Recombinant (E.coli)

Lyophilizate, sterile-filtered

Cat.No.     RP1002                       10 μg                 store at -20 ℃

Description:  Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17397 Dalton.

Source:        Escherichia coli.

Purity:         Greater than 95.0% as determined by SDS-PAGE.

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1EU/μg) as determined by the LAL method.

Biological Activity: The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be less than 0.1 ng/ml, corresponding to a specific activity of 3 x 10⁶ Units/mg.

Amino acid sequence: GSMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Formulation & storage: It was lyophilized from sterile 10 mM sodium phosphate (pH7.4) and we recommend that the powder be reconstituted in sterile 10 mW-cm H2O. Upon reconstitution, FGF2 should be stored at 4°C. For long- term storage at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Freeze-thaw cycles should be avoided.

Usage:  The product is for research only and may not be allowed for usage as drugs, agricultural or pesticidal products, food additives or household chemicals.

ArkOne Biotechnologies, Inc. is a global distributor of high quality biochemical reagents and lab research tools for protein chemistry, molecular biology, and cell biology including a series of DNA vectors, purified proteins of high quality, and media for cell culture. The pre-selected media are suitable for stem cell culture, especially for those mammalian stem cells isolated from bone marrow, umbilical cord, and adipose tissues. By using these media, cells can be quickly expanded with minimal differentiation.The serum media contain essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and pre-selected fetal bovine serum and other supplements. The serum-free media contain defined animal-free components and key cytokine supplements, targeting clinical applicaiton. We also provide packaging services for a variety of viruses including intergrative viruses and non-integrative viruses of high titer. Integrative viruses are suitable for rapid generation of stable cell lines. These viruses include retrovirus and classical lentivirus. Non-integrative viruses include modified lentivirus, adenovirus and adeno-associated viruses (AAV) with serotypes ranging from AAV2/1 to AAV2/9. Non-integrative viruses can serve as excellent options for transient transfection as well as for gene delivery treatment (http://).