Recombinant Trypsin
Catalog Number: RPT0201
CAS: 9002-07-7
EC: 3.4.21.4
Storage temperature: 2-8℃
Source: Recombinant trypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
2.MAIN FEATURES
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Source
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Recombinant E. coli
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Appearance
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White or off white, or yellowish powder
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Specific activity
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≥3800 USP units/mg pro.
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Purity(SDS-PAGE)
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Single major band
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Molecular Weight(SDS-PAGE)
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24.0±2.4 kDa
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UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant trypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
- Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
- Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
- Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as in insulin and vaccine production.
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
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