4-Methylumbelliferyl α-L-Idopyranosiduronic Acid 2-Sulfate Disodium Salt is a fluorogenic substrate in the assay of α-L-Iduronidase. 4-Methylumbelliferyl α-L-Idopyranosiduronic Acid 2-Sulfate Disodium Salt can be used for fluorimetric enzyme assay for the diagnosis of MPS II (Hunter disease). 4-Methylumbelliferyl α-L-Idopyranosiduronic Acid 2-Sulfate Disodium Salt has also been used as a substrate for iduronate-2-sulfatase in tests for Hunter disease.

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