1. General
Deoxynivalenol (DON) is one of several mycotoxins produced by certain Fusarium species that frequently infect corn, wheat, oats, barley, rice, and other grains in the field or during storage. Vomiting toxins have a very strong toxicity for both people and animals.It can cause human and animal vomiting, diarrhea, skin irritation, lack of apptite, neurological disorders, miscarriage, stillbirth,etc. Deoxynivalenol can occur in plant growth, harvest and processing, storage, transportation in the process.The timely detection of pollution sources is the best way to prevent Deoxynivalenol contamination.
2.Intended Use
A simple and efficient extraction and purification procedure for Deoxynivalenol was developed by means of the immunoaffinity column (IAC-SEP ® DON) as a cleanup tool．Deoxynivalenol content in corn, wheat, oats, barley, rice, and other grains are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast, simple, safe and highly accurate method for quantitatively measuring DON.
3.Principle
Samples are prepared by mixing with an extraction solution, blending and filtering.The extract is then applied to the Deoxynivalenol immunoaffinity column bound with specific antibodies to Deoxynivalenol．At this stage, the Deoxynivalenol binds to the antibody on the column.The column is then washed with water to remove the impurities.By passing methanol through the column, the Deoxynivalenol is removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.
ECALBO CO., LTD
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Address: HZAU, Nanhu Road, Wuhan city 430075, China.
Tel: +86 27 87745856
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E-mail: info@ecalbio.com bio-equip.cn
Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.
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ECALBIO CO.,LTD
HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com
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