1. General
Fumonisins are mycotoxins produced by the fungi Fusarium verticilliodes (= F.moniliforme) and F.proliferatum. Fusarium species are a frequent, almost universal, inhabitant of corn. Fumonisins are present in most corn samples tested. Fumonisin is thought to cause equine leukoencephalomalacia in horses, swine pulmonary edema, and human esophageal cancer.
2. Intended Use
A simple and efficient extraction and purification procedure for Fumonisins was developed by means of the immunoaffinity column (IAC-SEP®Fumo) as a cleanup tool．Fumonisins content in Rice, Corn, Wheat, Nuts, Spices, Peanuts Peanuts products, Vegetable oil, Feed samples are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring FumonisinB1,B2,B3.
3.Principle
Samples are prepared by mixing with an extraction solution, blending and filtering.The extract is then applied to the Fumonisins immunoaffinity column bound with specific antibodies to Fumonisins．At this stage, the Fumonisins bind to the antibody on the column.The column is then washed with water to remove the impurities.By passing methanol through the column, the Fumonisins are removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.
Wuhan Ecalbio Co., Ltd
Web: www.ecalbio.com
Address: HZAU, Nanhu Road, Wuhan city 430075, China.
Tel: +86 27 87745856
Fax: +86 27 87745856
E-mail: info@ecalbio.com bio-equip.cn
Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.
Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.
Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.
Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.
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ECALBIO CO.,LTD
HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com
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