Usage

Determination of contents of chondroitin sulfates by HPLC.

Research reagent (glycobiology, preparation of oligosaccharide libraries from chondroitin sulfates).

Degradation of hyaluronic acid to produce hyaluronic acid disaccharide.

General Information

Synonyms: Chondroitin sulfate lyase, chondroitin sulfate eliminase

Gene Source: Native Flavobacterium heparinum

EC Number: 4.2.2.5

CAS Number: 9047-57-8

MDL Number: MFCD00130796

Catalyzed Reaction: Chondroitinase AC cleaves, via na elimination mechanism, sulfated and non-sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosacchardie products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme is active on chondroitin sulfates A and C, chondroitin and hyaluronic acid, but is not active on dermatan sulfate (chondroitin sulfate B). 

Substrate Specificity: Chondroiting sulfates A and C, chondroitin, hyaluronic acid.  (The specific activity with chondroitin sulfate A is approx 1.5 times higher than the specific activity with chondroitin sulfate C.)

 

Properties

Molecular weight: 77kDa

Isoelectric point: 9.0 – 9.1

pH optimum for activity: 4.5 – 6 with chondroitin sulfate A , and 6 – 7 with chondroitin sulfate C

pH range for activity: 3.5 – 9 with chondroitin sulfate A, and 4.5 to 9 with chondroitin sulfate C

Optimal temperature range: 20 ℃ – 37 ℃

Purity: ≥ 90% by reversed phase HPLC analysis.

Standard: 1 IU and 5 IU.

Specific Activity: ≥ 100 IU/mg (substrate: chondroitin sulfate A). One international unit (IU) of chondroitinase AC is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from chondroitin sulfates A and C and hyaluronic acid per minute at 30 ℃ and pH 8.0.

Stability: 12 months at -20℃ in PBS.

 

Applications:

Chondroitinase AC is useful in many applications, for example:

As research reagent (glycosaminoglycan degradation).

For the preparation of di- and oligo-saccharides of chondroitin sulfates and the preparation of oligosaccharide libraries.

Degradation of hyaluronic acid.

Order Information

Catalog number: AG00-2780

ADHOC Intertek is a manufacturer and top supplier (over 80% market share the mainland China ) of detection and diagnostic reagents utilized in the production of heparin, LMWH, and chromogenic substrates during the quality control stage. We have accumulated over a decade of successful growth in China and internationally.