S-2238, which is specific to thrombin, is a short peptide covalently bound to pNA (4-nitroaniline). When attacked by thrombin, it gives up a free pNA which could be detected by a spectrophotometer at 405 nm. 

S-2238 is mainly used in anti-FIIa activity assay of heparin.

Applications:

S-2238 can be used in many applications, such as:

Anti-factor Xa activity to anti-factor IIa activity ratios of heparin and LMWHs

Anti-factor IIa activities of heparin and LMWHs

Activities of antithrombin and thrombin

Anti-factor IIa activities of heparin and LMWHs in human plasma

Production of kits for automated blood analyzers

Evaluation of potential anticoagulants from chemical synthesis, bacterial fermentation and animal tissues

Composition

S-2238 is white lyophizied powder, stored in vials.

Weight: 10mg and 25mg.

Each vial contains: S-2238 10 mg (15 μmol); mannitol 120 mg.

 

Physical and Chemical Properties:

Synonyms: S-2238, thrombin substrate 

CAS number: 113711-77-6

Molecular formula: H-D-Phe-Pip-Arg-pNA-2HCl

Molecular weight: 625.6 Da

Purity: ≥ 99% (HPLC)

Absorption coefficient: A405nm ≤ 0.120 [L/(g·cm)]

Solubility: > 10 mg/mL (in pure water)

 

Usage

Reconstitute the entire contents of a vial, dissolve it with 5 mL distilled water to get a solution with concentration of 3 mmol/mL S-2238.

Stability and Storage: 

The lyophilized powder of S-2238 could be preserved at 2 to 8 ℃ for at least 24 months.

The 3 mmol/mL S-2238 solution could be preserved at 2 to 8 ℃ for about 1 month.

 

Order Information:

Catalog number: AG00-0101

ADHOC Intertek is a manufacturer and top supplier (over 80% market share the mainland China ) of detection and diagnostic reagents utilized in the production of heparin, LMWH, and chromogenic substrates during the quality control stage. We have accumulated over a decade of successful growth in China and internationally.