Human antithrombin, prepared from human plasma, inactivates factor IIa (thrombin) and factor Xa, therefore can be used to determine the anti-FIIa and anti-FXa activities of heparin and LMWHs.

Applications

Human antithrombin can be used in many applications, such as:

1. Anti-factor Xa activity to anti-factor IIa activity ratios of heparin and LMWHs

2. Anti-factor IIa activities and anti-factor Xa activities of heparin and LMWHs

3. As a research reagent to investigate coagulation-related species

Composition

Human antithrombin is in form of white lyophilized powder stored in vials. Each vial contains: Human antithrombin 10 IU; Tris 60.5 mg.

Physical and Chemical Properties

Appearance: white lyophilized powder

Purity: electrophoretic purity

Molecular weight: about 56 kDa

E1cm1%(280 nm): about 10

Specific activity: not less than 6 IU/mg

Standard: 10 IU 

Usage

Reconstitute the entire contents of a vial, dissolve it with 10 mL distilled water to get a solution with concentration of 1 IU/mL human antithrombin, pH 7.4.

Stability and Storage: The lyophilized powder of human antithrombin could be preserved at 2 to 8 °C for 12 months. The 0.25 IU/mL human antithrombin solution could be preserved at 2 to 8 °C for about 1 month.

At Adhoc Intertek, we primarily focus on titer reagents of heparin for laboratory experiments and research. We are a professional vendor of detection reagents used in the production of heparin, LMWH, and chromogenic substrates during the quality control stage, also developing its own chain of professional equipment and tools (MPMS – multifunctional plasma mutagenesis system).