S-2238, which is specific to thrombin, is a short peptide covalently bound to pNA (4-nitroaniline). When attacked by thrombin, it gives up a free pNA which could be detected by a spectrophotometer at 405 nm. 

S-2238 is mainly used in anti-FIIa activity assay of heparin.

Applications:

S-2238 can be used in many applications, such as:

Anti-factor Xa activity to anti-factor IIa activity ratios of heparin and LMWHs

Anti-factor IIa activities of heparin and LMWHs

Activities of antithrombin and thrombin

Anti-factor IIa activities of heparin and LMWHs in human plasma

Production of kits for automated blood analyzers

Evaluation of potential anticoagulants from chemical synthesis, bacterial fermentation and animal tissues

Composition

S-2238 is white lyophizied powder, stored in vials.

Weight: 10mg and 25mg.

Each vial contains: S-2238 10 mg (15 μmol); mannitol 120 mg.

 

Physical and Chemical Properties:

Synonyms: S-2238, thrombin substrate 

CAS number: 113711-77-6

Molecular formula: H-D-Phe-Pip-Arg-pNA-2HCl

Molecular weight: 625.6 Da

Purity: ≥ 99% (HPLC)

Absorption coefficient: A405nm ≤ 0.120 [L/(g·cm)]

Solubility: > 10 mg/mL (in pure water)

 

Usage

Reconstitute the entire contents of a vial, dissolve it with 5 mL distilled water to get a solution with concentration of 3 mmol/mL S-2238.

Stability and Storage: 

The lyophilized powder of S-2238 could be preserved at 2 to 8 ℃ for at least 24 months.

The 3 mmol/mL S-2238 solution could be preserved at 2 to 8 ℃ for about 1 month.

 

Order Information:

Catalog number: AG00-0101

 

At Adhoc Intertek, we primarily focus on titer reagents of heparin for laboratory experiments and research. We are a professional vendor of detection reagents used in the production of heparin, LMWH, and chromogenic substrates during the quality control stage, also developing its own chain of professional equipment and tools (MPMS – multifunctional plasma mutagenesis system).