General Information

Catalog number: AG00-3367

Synonyms: Chondroitinase, chondroitin ABC lyase, chondroitin ABC eliminase

Gene Source: Proteus vulgaris

Express System: Escherichia coli

EC Number: 4.2.2.20

CAS Number: 9024-13-9

Catalyzed Reactions: Chondroitinase ABC cleaves, via and elimination mechanism, sulfated and non-sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopty at 232 nm. The enzyme is active on chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid. 

Substrate Specificity: Chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid. 

Properties

Molecular weight: 117.3 kDa

pH optimum for activity: 8.0

Optimal temperature range: 37 °C

Purity: ≥ 99% by reversed phase HPLC analysis.

Specific Activity: ≥ 100 IU/mg (substrate: chondroitin sulfate A).

Standard: 1 IU and 5 IU.

Unit Definition: One international unit (IU) of chondroitinase ABC is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from chondroitin sulfates A per minute at 30 °C and pH 8.0. 

Stability: Expiration is at least 12 months from manufacturing date, kept at -20 °C

Applications

Determination of contents of chondroitin sulfates by HPLC

Processing animal tissues before further investigations

Preparation of chondroitin and hyaluronic acid derived unsaturated disaccharides

Preparation of hyaluronic acid derived unsaturated disaccharide

Order Information

Catalog number: AG00-3667

At Adhoc Intertek, we primarily focus on titer reagents of heparin for laboratory experiments and research. We are a professional vendor of detection reagents used in the production of heparin, LMWH, and chromogenic substrates during the quality control stage, also developing its own chain of professional equipment and tools (MPMS – multifunctional plasma mutagenesis system).