Recombinant Carboxypeptidase B

Catalog Number:RCPB01

CAS: 9025-24-5
EC: 3.4.17.2

Storage temperature: 2-8℃

Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli 

Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

 

1.DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into  E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

 

2.MAIN FEATURES

Source	Recombinant E. coli
Appearance	White or off white, or yellowish powder
Specific activity	≥170 units/mg pro.
Purity(SDS-PAGE)	Single major band
Molecular Weight(SDS-PAGE)	33.8±3.4 kDa

UNIT DEFINITION：One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

 

3.RECOMMEND USAGE 

Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0. 

 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. 

It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

5.PRODUCT ADVANTAGE

①　Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.

②　Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

③　High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.

④　Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.

⑤　Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.

⑥　Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

6.PRODUCT USAGE

The production of recombinant insulin and its analogues;

the quality test of recombinant antibodies;

the determination of C-terminal amino acids of proteins;

the production of other recombinant polypeptides;

and the enzymatic synthesis of certain specific compounds.

 

7.RELATED PRODUCTS

Recombinant trypsin

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase

 

Shanghai Yaxin Biotechnology Co. Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins. Shanghai YaxinBio,located in Shanghai Juke Biotech Park, is ISO 9001 registered company since 2009.


Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.YaxinBio recombinant carboxypeptidase B and recombinant trypsin have no trace of other contaminating enzyme activities or enzyme inhibitors. They have been successfully used in the production process of recombinant insulin by several companies. Recombinant Trypsin can also be used in the process of cell culture.


Shanghai YaxinBio continuously strives to bring to its worldwide customers the best recombinant protein products. We will continue strengthen our product management and our internal system to meet the demands of biotech companies and scientists in the field of life sciences.