PRSSL1 encodes an arginine-specific serine protease and member of the peptidase S1 family of proteins. The encoded protein may undergo proteolytic activation before storage in azurophil granules, in neutrophil cells of the immune system. Following neutrophil activation, the protease is released into the pericellular environment, where it may play a role in defense against microbial pathogens.
Human Serine protease 57 (PRSSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSSL1 in samples. An antibody specific for PRSSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Serine protease 57 (PRSSL1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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