Pregnancy-specific protein B (PSPB), is secreted from binucleate trophoblast of the bovine conceptus as early as day 15 of pregnancy. This is the first indication that PSPB has a hormonal role in inducing GCP-2, an alpha chemokine that also is induced by interferon-tau during early pregnancy.
Human Pregnancy Specific Protein B (PSPB) ELISA Kit employs a two-site sandwich ELISA to quantitate PSG5 in samples. An antibody specific for PSG5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSG5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSG5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSG5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Pregnancy Specific Protein B (PSPB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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