Beta-agonist ELISA Kit detect the presence of Beta-agonist in samples of Animal tissue, urine and feed.
Introduction
Specifications
Principle: Direct enzyme-linked immunosorbent assay for the quantitative detect
Intended use: Detection of Beta-agonist
Specimen: Animal’s tissue, urine and feed.
Incubation Time: 30 min + 15 min
Standards: 0, 0.1, 0.3, 0.9, 2.7, 8.1 ng/mL
Detection Limit: Animal’s tissue 0.4 ppb / 0.2 ppb,Urine 0.1 ppb,Feed 5 ppb
Cross reaction:
Clenbuterol 100%,
Salbutamol 105%,
Tulobuterol 116.5%,
Pirbuterol 150%,
Carbuterol 191.5%,
Terbutaline 46.8%,
Mabuterol 40.1%,
Methyl-Clenbuterol 29.4%,
Cimaterol 6.4%,
Orciprenaline <7.8%
Recovery Rate: 90%±15% (pork liver 70%±15%)
Precision: Intra-assay CV<8% Inter-assay CV<15%
Shelf life: 12 months
Storage: 2- 8 °C. No freezing.
Packing size: 96 wells / kit
Advantages
Beta-agonist ELISA Kit enables the detection of very low residual level.
Beta-agonist ELISA Kit is a quick and accurate testing tool.
Beta-agonist ELISA Kit can be stored for 12 months between 2 and 30°C.
Instructions
Kit Component
- 1 X Microtiter plate (8 microwells X 12 removable strips)
- 6 X Standard solutions (1 mL each): 0 ng/ mL, 0.1 ng/ mL, 0.3 ng/ mL, 0.9 ng/ mL, 2.7 ng/ mL, 8.1 ng/ mL
- 1 X Standard solution (1 mL, for standard substance adding testing): 100 ng/mL
- 1 X Enzyme-linked conjugate (13 mL)
- 1 X Washing buffer concentrate (10 X , 50 mL)
- 1 X Substrate A (6 mL)
- 1 X Substrate B (6 mL)
- 1 X Stopping solution (6 mL)
- 1 X Product Manual
Materials Required But Not Provided
- ELISA Microtiter plate reader equipped with 450 nm filters
- 50 – 200 μL multichannel micropipette
- 50, 100 and 200 μL precision micropipette
- Microplate washer or squeeze bottle
- Homogenizer
- Centrifuge
- Centrifugal tubes
- Distilled water
- HCl, NaOH
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