The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The sonication shearing kit provides buffers to optimise the shearing conditions of your chromatin by sonication.
Isolation of good quality, suitably fragmented chromatin is the most important prerequisite for a successful ChIP assay. Chromatin can be sheared enzymatically or by mechanical methods such as sonication. Shearing by sonication can be advantageous for difficult to lyse cell types, It also provides random sample fragmentation preventing any bias.
The Chromatrap® Sonication Shearing Kit provides an excellent method for the preparation of high quality, ideally fragmented chromatin for ChIP analysis.
• The perfect solution to optimise the difficult to lyse sample preparations
• Kit supplies all reagents and buffers for up to 10 chromatin preparations
• Allows optimal shearing conditions to generate enough chromatin to perform up to 24 ChIPs using a standard Chromatrap® ChIP spin column kit or up to 96 IPs using the Chromatrap® 96 HT microplate kit
Chromatrap Sonication Shearing
Product Code : 500239
Kit Contents: 1.3M Glycine, Hypotonic Buffer, Lysis Buffer, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K and Proteinase K Stop Solution.
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