Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.
Please read this insert completely prior to using the product.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
【Intended Use】
This kit is used to assay the Vitamin E(VE)in the sample of Human’s serum, blood plasma, and other related tissue Liquid.
【Test principle】
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Vitamin E(VE)in samples. Add Vitamin E(VE) to monoclonal antibody Enzyme well which is pre-coated with Human Vitamin E(VE)monoclonal antibody, incubation; then, add Vitamin E(VE)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance Vitamin E(VE)of sample were positively correlated.
【Materials supplied in the Test Kit】
|
1
|
Standard(160nmol/ml)
|
0.5ml
|
7
|
Chromogen Solution A
|
6ml
|
|
2
|
Standard diluent
|
3ml
|
8
|
Chromogen Solution B
|
6ml
|
|
3
|
Microelisa Stripplate
|
12w×8s
|
9
|
Stop Solution
|
6ml
|
|
4
|
Str- HRP-Conjugate Reagent
|
6ml
|
10
|
Instruction
|
1
|
|
5
|
30×wash solution
|
20ml
|
11
|
Closure plate membrane
|
2
|
|
6
|
Biotin-VE Ab
|
1ml
|
12
|
Sealed bags
|
1
|
【Materials required but not supplied】
1. 37 ℃ incubator 2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips 4. Distilled water
5. Disposable tubes for sample dilution 6. Absorbent paper
Contact Rebecca Yan
Skype: span.biotech
Email: rebecca@spanbio.com
Tel: +86(755)89589611
Web:
www.spanbiotech.com
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