1. Principle
Kanamycin (KM) ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of Kanamycin in honey, tissue, milk.
The coupling antigens are coated on the micro-well stripes. The Kanamycinin the sample and the coupling antigens coated on the micro-well stripes compete for the anti-KM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Kanamycinin it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Kanamycin concentration in the sample.
2. Technical specification:
Sensitivity: 0.05ppb Detect time: Approx.75min
Detect limit:
Chicken, duck, pork, shrimp, fish ........ 2 ppb
Honey，milk ........................................ 2ppb
Cross-reaction rate:
Kanamycin............................................ 100%
Streptomycin ......................................< 1%
Dihydrostreptomycin..........................< 1%
Recovery rate:
85 ± 10%
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