1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Carbadox in the pork , liver, urine. The coupling antigen is pre-coated on the micro-well stripes. The Carbadox in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Carbadox antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Carbadox in it. This value is compared to the standard curve and the Carbadox residues is subsequently obtained.
2. Technical specifications
Sensitivity: 1.0ppb
Detection limit
Tissue.....................................2.5 ppb
Urine, serum........................2.0 ppb
Feed.......................................3.0 ppb
Recovery rate
Urine, serum.......................90±10%
Tissue.................................80±10%
Feed.....................................80±15%
Cross-reaction rate
Carbadox ...........................100%
MQCA...................................1%
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