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Recombinant Protein A
Recombinant Protein A
Place of Origin:
China
Brand:
YaxinBio
Model:
RSPA05
Price:
460$/g
Hits:
1673 
Updated:
10/10/2024
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    Recombinant Protein A

                             

    Catalog Number:RSPA05

    CAS:91932-65-9

    Storage temperature: 2-8℃

    Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli

     

    1.DESCRIPTION

    Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.

     

    2.MAIN FEATURES

    Appearance                  

    White or off white powder

    Solubility 

    Clear liquid

    Bioburden

    ≤ 1 cfu/ mg

    SDS-PAGE

    Single major band

    SDS-PAGE (MW)

    25000±1000 Daltons

    UV Spectrum 

    E275/250 ≥ 1.2

    E0.1% at 275nm = 0.17-0.23

    Purity (HPLC)

    ≥ 95%

     

    3.STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

    After dissolved with pure water, it should be stored under -20℃. 

    Stability of transport: The product is stable by blue ice insulation transport.

     

    4.PRODUCT ADVANTAGE

    1) Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.

    2) SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.

    3) The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.

    4) Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

    5) High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

    6) Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

    7) Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.

    8) Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    5.APPLICATION:

    The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

     

    6.RELATED PRODUCTS

    Recombinant carboxypeptidase B

    Sequencing grade trypsin

    Recombinant human trypsin

    Recombinant enterokinase

    bio-equip.cn
    Shanghai Yaxin Biotechnology Co. Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins. Shanghai YaxinBio,located in Shanghai Juke Biotech Park, is ISO 9001 registered company since 2009.


    Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.YaxinBio recombinant carboxypeptidase B and recombinant trypsin have no trace of other contaminating enzyme activities or enzyme inhibitors. They have been successfully used in the production process of recombinant insulin by several companies. Recombinant Trypsin can also be used in the process of cell culture.


    Shanghai YaxinBio continuously strives to bring to its worldwide customers the best recombinant protein products. We will continue strengthen our product management and our internal system to meet the demands of biotech companies and scientists in the field of life sciences.

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