Materials supplied in the Test Kit
|
1
|
Materials
provided with the kit
|
96 determinations
|
8
|
HRP-Conjugate reagent
|
6ml×1 bottle
|
|
2
|
User manual
|
1
|
9
|
Sample diluent
|
6ml×1 bottle
|
|
3
|
Closure plate membrane
|
2
|
10
|
Chromogen Solution A
|
6ml×1 bottle
|
|
4
|
Sealed bags
|
1
|
11
|
Chromogen Solution B
|
6ml×1 bottle
|
|
5
|
Microelisa stripplate
|
1
|
12
|
Stop Solution
|
6ml×1 bottle
|
|
6
|
Standard：72U/ml
|
0.5ml×1 bottle
|
13
|
wash solution
|
（20ml×30 fold）×1bottle
|
|
7
|
Standard diluent
|
1.5ml×1 bottle
|
|
|
|
Materials required but not supplied
1. 37 °C incubator
2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips
4. Distilled water
5. Disposable tubes for sample dilution
6. Absorbent paper
Summary Procedure
Preparing reagents, samples and standards
Add prepared samples and standards, antibodies labeled with enzyme, reacting 60 minutes at 37 °C
Plate washed five times, adding Chromogen solution A, B, reacting 10 minutes at 37 °C
Add stop solution
Measure the OD value within 10min
Package size:96T per box.
Validity&Storage:Six months(2-8°C)or Twelvemonths(-20°C)
bio-equip.cn
Sunglong Biotech, based in China, HangZhou, and have office in USA specialized in elisa kits, diagnostic reagent kit, antibody, food safety cards for life science research. The comprehensive product lines support applications in the study of all major life science research areas, including molecular biology, immunology, cell biology, proteomics and genomics research.
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