Principle
Fungal Identification Kit is based on PCR amplification of the fungal 26S rDNA D1/D2 region or the entire ITS sequence, according to sequencing analysis of the PCR amplified DNA fragment, fungal species can be identified. The 26S rDNA of fungi is divided into D1-D12 and other regions, among which the D1/D2 region has a sequence length of 500-600bp. The D1/D2 region sequences of most known yeast species model strains have been determined. The ITS, including ITS1 and ITS2, has a total length of 300-1000 bp.
This kit contains all the reagents needed for the amplification of the fungal 26S rDNA D1/D2 region and the ITS region. The PCR amplification primers D F/R are used to amplify the DNA fragment of the fungal 26S rDNA D1/D2 region, and the ITS F/R are used to amplify the DNA fragment of the fungal ITS region. At the same time, two sequencing primers are provided, including the forward sequencing primer Seq F and the reverse sequencing primer Seq R.
Wuhan Ecalbio Co., Ltd
Address: HZAU, Guanshan Road, Wuhan city 430075, China.
Tel: +86 27 87745856
Fax: +86 27 87745856
E-mail: info@ecalbio.com
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