1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of β-Lactams in the tissue, liver, honey, intestine, shrimp, fish, milk, etc. The coupling antigens are pre-coated on the micro-well stripes. The β-Lactams in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the β-Lactams receptors. After the addition of the antibodies and enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with β-Lactams concentration in the sample. This value is compared to the standard curve and the content of the corresponding β-Lactams is subsequently obtained.
2. Technical specifications
Sensitivity : 0.1 ppb Detect time: Approx. 75 min
Detection limit:
Tissue, liver, honey, shrimp, fish, milk ........... 2 ppb
Recovery rate:
Tissue, liver, honey, shrimp, fish, milk....... 85 ±10%
Cross-reaction rate:
Penicillin .......................100%
Ampicillin...................... 90 %
Amoxicillin.................... 80 %
Nafcillin……………… 110%
Amoxicillin …………… 80%
Piperacillin……………. 80%
Azlocillin...................... 90%
Cloxacillin .....................75%
Dicloxacillin...................50%
Oxacillin ....................... 50%
Metampicillin..................50%
Metampicillin..................30%
Carbenicillin....................10%
Ticarcillin ...................... 10%
Cephalosporins.............< 0.01%
ECALBIO CO.,LTD
Tel: +86 27 87745856
E-mail: info@ecalbio.com
Web: www.ecalbio.com
HZAU,Nanhu Road, Wuhan city ,China
bio-equip.cn
中文
