Porcine Foot And Mouth 3ABC Ab ELISA kit,Ecalbio_specification/price/image

TEST PRINCIPLE

Porcine FMD-3ABC Ab ELISA Test Kit is an enzyme immunoassay designed to detect the presence of

antibody to FMD-3ABC in the swine serum. A microtitration format has been configured by coating

FMD-3ABC antigen in alternating columns on the plate. Upon incubation of the test sample

in the coated well, antibody specific to FMD 3ABC forms a complex with the coated viral antigens.

After washing away unbound material from the wells, an anti-porcine: horseradish peroxidase conjugate

is added that binds to any porcine IgG antibody attached in the wells.

In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen,

3,3’,5,5-tetramethylbenzidine (TMB), are added to the wells. Stop the reaction by adding stop solution.

Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength if available.

Enzyme immunoassays for the qualitative of IgG antibodies against FMD-3ABC in pig serum or plasma.

2 Negative control 1 tube

3 Positive control 1 tube

4 Enzyme Conjugate 1 bottle

5 Washing buffer 2 bottle

6 Substrate A 1 bottle

7 Substrate B 1 bottle

8 Stop solution 1 bottle

9 Sample diluent 2 bottle

Storage

At 2-8℃,12 months.

Manufacturer

Wuhan Unibiotest Co., Ltd.

NO1 ,miaoshan medical zone, Wuhan ,China 430000

Tel: +86-27-81329156

MATERIALS SUPPLIED

1 Microtiter Plate 1 pieces (96 wells)