The kit applies improved guanidine thiocyanate-phenol one-step method to lyse samples and inactivate ribonucleases，then genomic DNA and 18 and 28s RNA are removed by the first spin-column. Then microRNA (including miRNA, snRNA,and other RNA less than 200bp) is absorbed by the second spin-column. After a serial of elution-centrifugation steps to remove cellular metabolite and proteins etc, finally microRNA will be eluted from silica membrane using low salt RNase-free water.
II. Features
1．The silica membranes in the spin-column are produced by the world-famous company. There is little variation among different spin-columns, so repeatability of tests is reliable!
2．MicroRNA can be eluted from silica membrane without ethanol precipitation and dissolve.
3．Multi-elution can ensure high-purity microRNA.
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