DNzol:DNA Rapid Extraction Reagents
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SKU
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Product
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Preps
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Price(USD)
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DP3001
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DNzol Genomic DNA Rapid Extraction Reagents
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50
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53
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DP3002
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100
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95
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Description
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DNAzol reagents are pre-mixed, ready-to-use reagents for the extraction of genomic DNA.
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Primary principle
DNAzol Reagent is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol Reagent procedure is based on the use of a novel guanidine-detergent lysing solution which allows for selective precipitation of DNA from a cell lysate. Developed by Chomczynski, DNAzol Reagent is an advanced DNA isolation reagent that combines both reliability and efficiency with simplicity of the isolation protocol. The DNAzol Reagent protocol is fast and permits isolation of genomic DNA from a large number of samples of small or large volumes. During the isolation, a biological sample is lysed (or homogenized) in DNAzol Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, DNA is solubilized in water or 8 mM NaOH.
Feature and benefits
The procedure can be completed in 10-30 min with DNA recovery of 70-100%.
The isolated DNA can be used without additional purification for applications such as PCR, restriction digestion, southern blot, and dot blot hybridization
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