Sepax BR-C18 Columns,Sepax Technologies, Inc._specification/price/image

Product Detail
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Description

Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, 
BR-C18 bonded phases have been innovatively and specially designed to ensure maximum 
surface coverage and full end-capping, which leads to carbon content as high as 19.0%. 
The bonding chemistry is completely controlled that results in very reliable column-to-column
 reproducibility. The maximum surface coverage allows BR-C18 to have exceptional stability, 
resulting in high pH stability in the range of 1.5 to 10.5.
Packing

Silica:	Spherical, high purity (<10 ppm metals)
Pore size:	120Å
Particle size:	3, 5, and 10 µm
Pore volume:	1.0 ml/g
Surface area:	350 m²/g
Phase structure:	Monomeric and fully endcapped
% Carbon:	19.0%

Characteristic

Highly controlled chemistry of monolayer formation and end-capping
Extremely high column-to-column reproducibility
High selectivity and efficiency for separations
pH range 1.5-10.5
Suitable for separations of acidic, neutral and basic compounds, peptides, and proteins

Column Stability at Alkali Conditions

BR-C18 uses full coverage bonded silica packing, which allows high stability at high pH. Figure below shows reproducible retention time for a test compound: toluene after 18,000 column volume runs in a mobile phase of 55% acetonitrile and 45% water at pH 10. Such high stability allows BR-C18 well suited for validation of various analytes at alkali conditions. The proprietary bonding chemistry for BR-C18 allows achieving high selectivity and high efficiency separation.

A BR-C18 column was operated at pH 10.

Conditions
Column:	BR-C18, 3 µm, 4.6×50 mm
Mobile phase:	10 mM ammonium bicarbonate buffer in 55%ACN/45%H₂O
Temperature:	Ambient
Flow rate:	0.5 mL/min
Detector:	UV 254 nm
Sample:	Toluene