Sepax Antibodix Columns,Sepax Technologies, Inc._specification/price/image

Product Detail
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Description

Antibodix columns are specially designed for high resolution, high efficiency and high recovery separations of antibodies. The packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead. The non-porous resin has particle size of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. On the top of the hydrophilic layer, weak cation-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.
Packing

Support:	High cross-linked, spherical PS/DVB beads
Particle size:	1.7, 3, 5 and 10 µm
Pore size:	non-porous
Phase structure:	weak cation exchanger with carboxylate functional groups chemically bonded and hydrophilic

Characteristic

Highly cross-linked non-porous PS/DVB particle as the support
Uniform particle size
Particle size availability: 1.7, 3, 5, and 10 µm
Weak-cation exchange chemistry
Hydrophilic surface specially designed for elimination of non-specific bindings
High separation efficiency, selectivity and resolving power
High lot-to-lot reproducibility (link to below header)
High loading capacity (link to below header)
High recovery
High mechanical stability with pressure tolerance higher than 8,000 psi for 5 and 10 µm, and 10,000 psi for 1.7 and 3 µm
High pH stability: 2-12
Well suited for UPLC and regular HPLC systems
Complete selection for analytical, semi-preparative and preparative separations
Applications for proteins, especially antibodies

Chromatogram of a protein mixture separated by Antibodix NP5, 7.8×50mm

Conditions
Column:	Antibodix NP5 (5 µm, 7.8×50 mm)
Mobile phase:	A: 10 mM phosphate buffer, pH 6 B: A + 1.0 M NaCl
Gradient:	10-45%B (21 min)
Flow rate:	1.5 mL/min
Temperature:	Ambient
Injection volume:	10 µL
Sample:	1. Cytochrome C 2. Lysozyme 3. Ribonuclease A

Separation of MAb on Antibodix NP1.7 (1.7 µm, 4.6×50 mm)

Conditions
Column:	Antibodix NP1.7 (1.7 µm, 4.6×50 mm)
Mobile phase:	A. 10 mM phosphate buffer, pH 7.5 B. A + 100 mM NaCl
Gradient:	15-55% B (30 min)
Flow rate:	0.3 mL/min
Detector:	UV 214 nm
Temperature:	Ambient
Injection volume:	5 µL
Sample:	Monoclonal antibody (5 mg/mL)

High lot to lot reproducibility

Sample 1. Protein mixtures

Conditions
Column:	Antibodix NP10 (10 µm, 4.6×250 mm)
Mobile phase:	A: 10 mM phosphate buffer, pH 7.5 B: A + 100 mM NaCl
Gradient:	15-55%B (30 min)
Flow rate:	0.8 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	5 µL
Sample:	1. Cytochrome C (5 mg/mL) 2. Lysozyme (5 mg/mL) 3. Ribonuclease A (5 mg/mL)

Sample 2. Monoclonal antibodies

Conditions
Column:	Antibodix NP10 (10 µm, 4.6×250 mm)
Mobile phase:	A. 10 mM phosphate buffer, pH 7.5 B. A + 100 mM NaCl
Gradient:	15-55% B (30 min)
Flow rate:	0.8 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	5 µL
Sample:	Monoclonal antibody (5 mg/mL)

High Loading

Conditions
Column:	Antibodix NP10 (10 µm, 4.6×250 mm)
Mobile phase:	A: 10 mM phosphate buffer, pH 6 B: A + 1.0 M NaCl
Gradient:	10-100%B (25 min)
Flow rate:	0.8 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Sample:	1. Aprotinin (3.3 mg/mL) 2. Lysozyme (5.6 mg/mL) 3. Ribonuclease (3.0 mg/mL)